tag:blogger.com,1999:blog-6181073719515632715.post4831372651805965906..comments2024-02-27T06:57:49.831-05:00Comments on Democratizing Cell Technologies: Current Bottlenecks in MSC Research: MSC Misconceptions - Part IIJon Rowleyhttp://www.blogger.com/profile/10634518820328346758noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-6181073719515632715.post-25632573676335301912016-05-12T02:35:21.347-04:002016-05-12T02:35:21.347-04:00This information is very usefull... thanks for sha...This information is very usefull... thanks for sharing....<br /><a href="http://www.chennaienggcolleges.com/colleges-and-universities/" rel="nofollow">Engineering College Admissions 2016</a>Latha Priyahttps://www.blogger.com/profile/04526967133087119008noreply@blogger.comtag:blogger.com,1999:blog-6181073719515632715.post-5247381649862039302014-11-26T02:19:59.353-05:002014-11-26T02:19:59.353-05:00Hi
Thanks for the nice article
i have doubts rega...Hi <br />Thanks for the nice article<br />i have doubts regarding MSCs culture and calculating the PDT<br />1) How to calculate the PDT for primary cultures, since the MSCs harvested from the tissues (Bone marrow/adipose etc) contains more contaminated cells and getting accurate seeding density is not possible. <br />any inputs regarding, method to count the primary cells will be useful for my research.<br />2) Even if you add the same seeding density during primary culture, i have seen the difference between the samples. some cells get senescent in p1 itself. how to manage this kind of samples. <br />Note-I used automated cell counter (horiba abx) and the samples are harvested from young patients.<br />karthikhttps://www.blogger.com/profile/18076693858923080963noreply@blogger.com